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45-023 (0.01ug/ml) staining of Molt-4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-023 (0.01ug/ml) staining of Molt-4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BIRC5 Antibody

CATALOG NUMBER: 45-023

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Cat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: CVRRAIEQLAAMD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 18kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 16.4kDa according to NP_001159.2 and 18.6kDa according to NP_001012271.1). Recommended concentration: 0.01-0.5ug/ml.
Specificity:
This antibody is expected to recognize the reported isoforms 1 (NP_001159.2) and isoform 3 (NP_001012271.1).
Positive Control 1:
Cat. No. 1206 - MOLT4 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BIRC5
Additional Names:
BIRC5, survivin, API4, EPR-1, baculoviral IAP repeat-containing 5 (survivin), apoptosis inhibitor 4, baculoviral IAP repeat-containing 5, baculoviral IAP repeat-containing protein 5, survivin variant 3 alpha
Protein Accession Number:
NP_001159.2,NP_001012271.1
Protein Gi Number:
59859878
Ncbi Gene Id Number:
332
Background Reference 1:
Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC. Control of apoptosis and mitotic spindle checkpoint by survivin. Nature. 1998 Dec 10;396(6711):580-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-023

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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