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45-024 staining (0.3ug/ml) of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-024 staining (0.5ug/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-024 staining (0.3ug/ml) of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BIRC7 Antibody

CATALOG NUMBER: 45-024

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RAPVRSRVRT
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 30kDa and 35kDa in lysates of cell line MOLT4, and 30kDa in lysates of Daudi, Jurkat and K562. These bands correspond to the reported isoforms with calculated MW of 32.8kDa according to NP_647478.1 and 30.9kDa according to NP_071444.1. Recommended concentration: 0.3 - 1 ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (as represented by NP_647478.1 and NP_071444.1)
Positive Control 1:
Cat. No. 1206 - MOLT4 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BIRC7
Additional Names:
BIRC7, LIVIN, baculoviral IAP repeat-containing 7 (livin), KIAP, MLIAP, mliap, ML-IAP, livin inhibitor-of-apotosis, kidney inhibitor of apoptosis protein, melanoma inhibitor of apoptosis protein, baculoviral IAP repeat-containing protein 7, RNF50, livin inhibitor of apoptosis
Protein Accession Number:
NP_647478.1,NP_071444.1
Protein Gi Number:
21536421
Ncbi Gene Id Number:
79444
Background Reference 1:
Kasof GM, Gomes BC. Livin, a novel inhibitor of apoptosis protein family member. J Biol Chem. 2001 Feb 2;276(5):3238-46. Epub 2000 Oct 9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-024

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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