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Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 μg/mL.
Immunofluorescence of Bit1 in 3T3 cells with Bit1 antibody at 20 μg/mL.
Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Trial Size

Bit1 Antibody

CATALOG NUMBER: 3603

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Bit1 antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human Bit1.
The immunogen is located within amino acids 20 - 70 of Bit1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 3:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification:
Bit1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Bit1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bit1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PTRH2
Additional Names:
Bit1 Antibody: BIT1, PTH2, CGI-147, BIT1, Peptidyl-tRNA hydrolase 2, mitochondrial, Bcl-2 inhibitor of transcription 1, PTH 2
Protein Accession Number:
NP_057161
Protein Gi Number:
7706351
Ncbi Gene Id Number:
51651
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bit1 Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Background Reference 1:
Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3603

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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