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42-447 (0.1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-447 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-447 (0.1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

BLK Antibody

CATALOG NUMBER: 42-447

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: QWSPLKVSAQDKD-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Thymus, Spleen and Lymph Node lysates (calculated MW of 57.7kDa according to NP_001706.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1314 - Human Thymus Tissue Lysate
Positive Control 2:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 3:
Cat. No. 1369 - Human Lymph node Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BLK
Additional Names:
BLK, B lymphoid tyrosine kinase, MGC10442, MODY11, BLK nonreceptor tyrosine kinase, b lymphocyte kinase, p55-Blk, tyrosine-protein kinase Blk
Protein Accession Number:
NP_001706.2
Protein Gi Number:
33469982
Ncbi Gene Id Number:
640

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-447

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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