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Western blot analysis of BLM Antibody in HL-60 cell line lysates (35ug/lane)
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of BLM Antibody in HL-60 cell line lysates (35ug/lane)

BLM Antibody

CATALOG NUMBER: 61-686

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1054-1082 amino acids from the C-terminal region of human BLM.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
159 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BLM
Additional Names:
Bloom syndrome protein, DNA helicase, RecQ-like type 2, RecQ2, RecQ protein-like 3, BLM, RECQ2, RECQL3
Protein Accession Number:
P54132
Protein Gi Number:
1705486
Ncbi Gene Id Number:
641
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BLM is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities.
Background Reference 1:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Background Reference 2:
Frank, B., et al. Carcinogenesis 31(3):442-445(2010)
Background Reference 3:
Ouyang, K.J., et al. PLoS Biol.(12), E1000252 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-686

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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