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Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody. The lane on the right is treated with the antigen-specific peptide.

Bloom Syndrome (phospho Thr99) Antibody

CATALOG NUMBER: 79-803

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Bloom Syndrome (phospho Thr99) antibody was raised against a peptide sequence around phosphorylation site of threonine 99 (Q-E-T (p)-Q-R) derived from Human Bloom Syndrome.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.
Predicted Molecular Weight:
159 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
BLM
Additional Names:
RECQ2, RECQL3, type 2, EC 3.6.1, RecQ protein-like 3
Protein Accession Number:
NP_000048.1
Protein Gi Number:
1705486
Ncbi Gene Id Number:
641
Background:
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).
Background Reference 1:
Ellis N.A., Cell 83:655-666(1995).
Background Reference 2:
Karow J.K., J. Biol. Chem. 272:30611-30614(1997).
Background Reference 3:
Pichierri P., EMBO J. 23:3154-3163(2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-803

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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