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43-567 (0.1ug/ml) staining of K562 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-567 (0.3ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-567 (2ug/ml) staining of Pig Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-567 (0.1ug/ml) staining of K562 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BMI1 Antibody

CATALOG NUMBER: 43-567

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-SHQRDGLTNAGELES
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in nuclear lysates of cell lines K562 and NIH3T3 (calculated MW of 36.9kDa according to NP_005171.4). This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev. 2006 Aug 1;20(15):2110-20. PMID: 16882984). Recommended concentration: 0.1-0.3ug/ml.Approx 37kDa band observed in Pig Bone Marrow lysates (calculated MW of 36.9kDa according to NP_001272900.1 ). Recommended concentration: 1-3ug/ml.
Predicted Molecular Weight:
Approx 40kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BMI1
Additional Names:
BMI1, BMI1 polycomb ring finger oncogene, FLVI2/BMI1, PCGF4, RNF51, B lymphoma Mo-MLV insertion region 1 homolog, flvi-2/bmi-1, murine leukemia viral (bmi-1) oncogene homolog, polycomb complex protein BMI-1, polycomb group RING finger protein 4, polycomb
Protein Accession Number:
NP_005171.4
Protein Gi Number:
22258801
Ncbi Gene Id Number:
648
Background Reference 1:
Cho JH, Dimri M, Dimri GP.A positive feedback loop regulates the expression of polycomb group protein BMI1 via WNT signaling pathway.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-567

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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