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Western blot analysis of BMI1 Antibody antibodyin 293 cell line lysates (35ug/lane)
Western blot analysis in K562 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with BMI1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of BMI1 Antibody antibodyin 293 cell line lysates (35ug/lane)

BMI1 Antibody

CATALOG NUMBER: 61-406

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Chicken, Bovine
Immunogen:
This BMI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the C-terminal region of human BMI1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BMI1
Additional Names:
Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51
Protein Accession Number:
P35226
Protein Gi Number:
22258801
Ncbi Gene Id Number:
1.00533E+11
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BMI is a component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2. BMI cooperates with the MYC oncogene to produce B-lymphomas.
Background Reference 1:
Smith, K.S., et al., Mol. Cell 12(2):393-400 (2003). Xia, Z.B., et al., Proc. Natl. Acad. Sci. U.S.A. 100(14):8342-8347 (2003). Itahana, K., et al., Mol. Cell. Biol. 23(1):389-401 (2003). Dimri, G.P., et al., Cancer Res. 62(16):4736-4745 (2002). Bardos, J.I., et al., J. Biol. Chem. 275(37):28785-28792 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-406

List Size:
400 ul

List Price:

$495.00

Datasheet
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