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43-642 (1ug/ml) staining of Human Heart (A) and Kidney (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-642 (2ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-642 (2ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-642 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-642 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-642 (1ug/ml) staining of Human Heart (A) and Kidney (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

BMP1 Antibody

CATALOG NUMBER: 43-642

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DTIVPKYEVNGVK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Heart and Human Kidney lysates and approx 100kDa band observed in Rat Heart lysates and in cell lysates of cell line NIH3T3 (calculated MW of 111kDa according to Human NP_006120.1, 97.7kDa according to Mouse XP_011243231.1, and 93.5kDa according to Rat XP_006252347.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Heart and Kidney for this particular batch.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Placenta. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001190.1; NP_006120.1).
Predicted Molecular Weight:
Approx 110kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BMP1
Additional Names:
BMP1, bone morphogenetic protein 1, OI13, PCOLC, PCP, PCP2, TLD, mammalian tolloid protein, procollagen C-endopeptidase, procollagen C-proteinase
Protein Accession Number:
NP_001190.1,NP_006120.1
Protein Gi Number:
13124688
Ncbi Gene Id Number:
649
Background Reference 1:
Wermter C, Höwel M, Hintze V, Bombosch B, Aufenvenne K, Yiallouros I, Stöcker W.The protease domain of procollagen C-proteinase (BMP1) lacks substrate selectivity, which is conferred by non-proteolytic domains.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-642

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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