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Western blot analysis of extracts of various cell lines, using BMP5 antibody (23-558) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat lung using BMP5 antibody (23-558) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using BMP5 antibody (23-558) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using BMP5 antibody (23-558) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using BMP5 antibody (23-558) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using BMP5 antibody (23-558) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using BMP5 antibody (23-558) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

BMP5 Antibody

CATALOG NUMBER: 23-558

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 317-454 of human BMP5 (NP_066551.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
H460
Positive Control 2:
LO2
Positive Control 3:
SW620
Positive Control 4:
293T
Positive Control 5:
mouse liver
Positive Control 6:
mouse small intestine
Predicted Molecular Weight:
Observed: 52kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
BMP5
Additional Names:
BMP5, bone morphogenetic protein 5, MGC34244,
Ncbi Gene Id Number:
653
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. These proteins are synthesized as prepropeptides, cleaved, and then processed into dimeric proteins. This protein may act as an important signaling molecule within the trabecular meshwork and optic nerve head, and may play a potential role in glaucoma pathogenesis. This gene is differentially regulated during the formation of various tumors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-558

List Size:
100 uL

List Price:

$436.00

Datasheet
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