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45-334 (1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-334 (1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BMPR1A Antibody

CATALOG NUMBER: 45-334

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-KSDSDQKKSEN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in lysates of cell lines HeLa and HepG2 (calculated MW of 60.2kDa according to NP_004320.2). Recommended concentration: 1-3ug/ml.An additional band of 20kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BMPR1A
Additional Names:
BMPR1A, bone morphogenetic protein receptor, type IA , HGNC:1076, ACVRLK3, ALK3, activin A receptor, type II-like kinase 3
Protein Accession Number:
NP_004320.2
Protein Gi Number:
41349437
Ncbi Gene Id Number:
657
Background Reference 1:
Keller S, Nickel J, Zhang JL, Sebald W, Mueller TD. Molecular recognition of BMP-2 and BMP receptor IA. Nat Struct Mol Biol. 2004 May;11(5):481-8. Epub 2004 Apr 4.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-334

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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