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Western blot analysis of extracts of various cell lines, using BNIP3 antibody (19-791) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse kidney using BNIP3 antibody (19-791) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (19-791) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using BNIP3 antibody (19-791) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using BNIP3 antibody (19-791) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using BNIP3 antibody (19-791) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

BNIP3 Antibody

CATALOG NUMBER: 19-791

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human BNIP3 (NP_004043.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
NCI-H460
Positive Control 3:
Jurkat
Positive Control 4:
SH-SY5Y
Positive Control 5:
Mouse kidney
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 22-70kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
BNIP3
Additional Names:
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, BNIP3, NIP3
Ncbi Gene Id Number:
664
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-791

List Size:
100 uL

List Price:

$436.00

Datasheet
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