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Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

BOLL Antibody

CATALOG NUMBER: 58-408

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This BOLL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human BOLL.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BOLL
Additional Names:
Protein boule-like, BOLL, BOULE
Protein Accession Number:
Q8N9W6
Protein Gi Number:
44887719
Ncbi Gene Id Number:
66037
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BOLL belongs to the DAZ gene family required for germcell development. It encodes an RNA-binding protein which is moresimilar to Drosophila Boule than to human proteins encoded by genesDAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like).Loss of this gene function results in the absence of sperm in semen(azoospermia). Histological studies demonstrated that the primarydefect is at the meiotic G2/M transition.
Background Reference 1:
Chen, P., et al. J. Androl. 31(2):163-168(2010)
Background Reference 2:
Kee, K., et al. Nature 462(7270):222-225(2009)
Background Reference 3:
Lin, Y.M., et al. J. Clin. Endocrinol. Metab. 94(7):2650-2657(2009)
Background Reference 4:
Bilguvar, K., et al. Nat. Genet. 40(12):1472-1477(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-408

List Size:
400 ul

List Price:

$495.00

Datasheet
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