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Western blot analysis of Bora in mouse brain tissue lysate with Bora antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 μg/mL.
Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 μg/mL.
Western blot analysis of Bora in mouse brain tissue lysate with Bora antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Bora Antibody

CATALOG NUMBER: 5107

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Bora antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Bora.
The immunogen is located within amino acids 410 - 460 of Bora.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Bora antibody can be used for detection of Bora by Western blot at 1-2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Bora Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Bora Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bora antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BORA
Additional Names:
Bora Antibody: C13orf34, C13orf34, Protein aurora borealis, HsBora
Protein Accession Number:
Q6PGQ7
Protein Gi Number:
74737659
Ncbi Gene Id Number:
79866
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bora Antibody: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.
Background Reference 1:
Berdnik D and Knoblich JA. Drosophila Aurora A is required for centrosome maturation and Actin-dependent asymmetric protein localization during mitosis. Curr. Biol.2002; 12:640-647.
Background Reference 2:
Wiese C and O'Brien LL. What's so Bor(a)ing about Aurora A activation? Dev. Cell2006; 11:133-134.
Background Reference 3:
Hutterer A, Berdnik D, Wirtz-Peitz F, et al. Mitotic activation of the kinase Aurora A requires its binding partner Bora. Dev. Cell2006; 11:147-157.
Background Reference 4:
Fu J, Bian M, Jiang Q, et al.. Roles of Aurora kinases in mitosis and tumorigenesis. Mol. Cancer Res.2007; 5:1-10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5107

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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