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Western Blot at 1:2000 dilution Lane 1: MDA-MB-453 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: MDA-MB-453 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

BPGM Antibody

CATALOG NUMBER: 56-539

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This BPGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-259 amino acids from the C-terminal region of human BPGM.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BPGM
Additional Names:
Bisphosphoglycerate mutase, BPGM,3-bisphosphoglycerate mutase, erythrocyte,3-bisphosphoglycerate synthase,3-diphosphoglycerate mutase, DPGM, BPG-dependent PGAM, BPGM
Protein Accession Number:
P07738
Protein Gi Number:
130350
Ncbi Gene Id Number:
669
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
2,3-diphosphoglycerate (2,3-DPG) is a small molecule foundat high concentrations in red blood cells where it binds to anddecreases the oxygen affinity of hemoglobin. This gene encodes amultifunctional enzyme that catalyzes 2,3-DPG synthesis via itssynthetase activity, and 2,3-DPG degradation via its phosphataseactivity. The enzyme also has phosphoglycerate phosphomutaseactivity. Deficiency of this enzyme increases the affinity of cellsfor oxygen. Mutations in this gene result in hemolytic anemia.Multiple alternatively spliced variants, encoding the same protein,have been identified.
Background Reference 1:
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Background Reference 2:
Wang, Y., et al. J. Biol. Chem. 281(51):39642-39648(2006)
Background Reference 3:
Wang, Y., et al. J. Biol. Chem. 279(37):39132-39138(2004)
Background Reference 4:
Fujita, T., et al. J. Biochem. 124(6):1237-1244(1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-539

List Size:
400 ul

List Price:

$495.00

Datasheet
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