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Western blot analysis of BRAL1 in human brain tissue lysate with BRAL1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemical staining of human brain tissue using BRAL1 antibody at 2.5 μg/mL.
Immunofluorescence of BRAL1 in Human Brain cells with BRAL1 antibody at 20 μg/mL.
Western blot analysis of BRAL1 in human brain tissue lysate with BRAL1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

BRAL1 Antibody

CATALOG NUMBER: 4501

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
BRAL1 antibody was raised against a 12 amino acid synthetic peptide from near the center of human BRAL1.
The immunogen is located within amino acids 130 - 180 of BRAL1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
BRAL1 antibody can be used for the detection of BRAL1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
BRAL1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BRAL1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BRAL1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HAPLN2
Additional Names:
BRAL1 Antibody: BRAL1, Hyaluronan and proteoglycan link protein 2, Brain link protein 1
Protein Accession Number:
NP_068589
Protein Gi Number:
11141887
Ncbi Gene Id Number:
60484
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BRAL1 Antibody: BRAL1 is a member a superfamily consisting of several highly homologous hyaluronan and proteoglycan binding link proteins. BRAL1 is predominantly expressed in brain tissue and spinal cord. Like other members in the link-module superfamily, BRAL1 contains an immunoglobulin-like fold and two proteoglycan tandem repeats (PTRs). Its mRNA expression pattern is similar to other lectican proteoglycans, suggesting that BRAL1 may act to stabilize the binding between the extracellular matrix molecule hyaluronan and brevican. Immunostaining of mouse brain showed BRAL1 expression at P20 in the white matter of the developing cerebellum and in myelinated fiber tracts in the adult brain, suggesting that expression starts when axonal myelination occurs.
Background Reference 1:
Spicer AP, Joo A, and Bowling Jr. RA. A hyaluronan binding link protein gene family whose members are physically linked adjacent to chrondroitin sulfate proteoglycan core protein genes. J. Biol. Chem.2003; 278:21083-91.
Background Reference 2:
Hirakawa S, Oohashi T, Su W-D, et al. The brain link protein-1 (BRAL1): cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain. Biochem. Biophys. Res. Comm.2000; 276:982-9.
Background Reference 3:
Oohashi T, Hirakawa S, Bekku Y, et al. Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult mouse central nervous systems. Mol. Cell Neurosci.2002; 19:43-57.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4501

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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