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Western blot analysis of lysed extracts from HeLa and HT29 cells using BRCA1 (Ab-1423).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1 (Ab-1423).
Western blot analysis of lysed extracts from HeLa and HT29 cells using BRCA1 (Ab-1423).

BRCA1 (Ab 1423) Antibody

CATALOG NUMBER: 79-376

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
BRCA1 (Ab-1423) antibody was raised against a peptide sequence around aa.1421~1425 (H-G-S-Q-P) derived from Human BRCA1.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total BRCA1 protein.
Predicted Molecular Weight:
220 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
BRCA1
Additional Names:
IRIS, PSCP, BRCAI, BRCC1, PNCA4, RNF53, BROVCA1, PPP1R53, NACP, PARK1
Protein Accession Number:
NP_009225.1
Protein Gi Number:
6552299
Ncbi Gene Id Number:
672
Background:
The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation
Background Reference 1:
Kim ST, et al. (2002) Genes Dev; 16 (5): 560-570Arlt MF, et al. (2004) Mol Cell Biol; 24 (15): 6701-6709Zhang J, et al. (2004) Mol Cell Biol; 24 (2): 708-718Greenberg RA, et al. (2006) Genes Dev; 20 (1): 34-46Tibbetts RS, et al. (2000) Genes Dev; 14 (23): 2989-3002

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-376

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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