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Antibody used in WB on Human Thymus at 0.2-1 ug/ml.
Antibody used in CHIP on HCT116 Cells.
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.

BRD4 Antibody

CATALOG NUMBER: 29-005

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BRD4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
BRD4 antibody can be used for detection of BRD4 by ELISA at 1:1562500. BRD4 antibody can be used for detection of BRD4 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate
Predicted Molecular Weight:
80 kDa, 152 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store BRD4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
BRD4
Additional Names:
BRD4, CAP, HUNKI, MCAP, HUNK1
Protein Accession Number:
NP_055114
Protein Gi Number:
7657218
Ncbi Gene Id Number:
23476
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting.The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t (15;19) (q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Background Reference 1:
Schweiger, M.R., (2006) J. Virol. 80 (9), 4276-4285.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-005

List Size:
100 ul

List Price:

$519.00

Datasheet
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