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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

BRD7 Antibody

CATALOG NUMBER: 28-997

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BRD7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
BRD7 antibody can be used for detection of BRD7 by ELISA at 1:312500. BRD7 antibody can be used for detection of BRD7 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
74 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store BRD7 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
BRD7
Additional Names:
BRD7, BP75, CELTIX1, NAG4
Protein Accession Number:
NP_037395
Protein Gi Number:
41350212
Ncbi Gene Id Number:
29117
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BRD7 is an activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. It induces dephosphorylation of GSK3B at 'Tyr-216'.
Background Reference 1:
Zhou, J., (2004) J. Cell. Physiol. 200 (1), 89-98.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-997

List Size:
100 ul

List Price:

$519.00

Datasheet
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