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BRDT Antibody

CATALOG NUMBER: 45-340

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QSDIMTMFENNFD
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 75kDa band in human testis lysate after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 108kDa according to NP_001717. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
Variants (NP_001717.2; NP_997072.1) encode the same protein.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BRDT
Additional Names:
BRDT, bromodomain, testis-specific, BRD6, OTTHUMP00000011809, OTTHUMP00000197577, cancer/testis antigen 9, testis-specific bromodomain protein
Protein Accession Number:
NP_001717.2,NP_997072.1
Protein Gi Number:
46399198
Ncbi Gene Id Number:
676
Background Reference 1:
Jones MH, Numata M, Shimane M. Identification and characterization of BRDT: A testis-specific gene related to the bromodomain genes RING3 and Drosophila fsh. Genomics. 1997 Nov 1;45(3):529-34.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-340

List Size:
0.1 mg

List Price:

$391.00

Datasheet
Shipping Info

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