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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis in Hela cell line lysates (35ug/lane).

BRF1 Antibody

CATALOG NUMBER: 57-998

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-342 amino acids from the Central region of human BRF1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
74 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BRF1
Additional Names:
Transcription factor IIIB 90 kDa subunit, TFIIIB90, hTFIIIB90, B-related factor 1, BRF-1, hBRF, TAF3B2, TATA box-binding protein-associated factor, RNA polymerase III, subunit 2, BRF1, BRF, GTF3B, TAF3B2, TAF3C
Protein Accession Number:
Q92994
Protein Gi Number:
20455319
Ncbi Gene Id Number:
2972
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of the three subunits of the RNApolymerase III transcription factor complex. This complex plays acentral role in transcription initiation by RNA polymerase III ongenes encoding tRNA, 5S rRNA, and other small structural RNAs. Thegene product belongs to the TF2B family. Two alternatively splicedvariants encoding different isoforms, that function at differentpromoters transcribed by RNA polymerase III, have been identified.Other transcript variants are possible, but their full-lengthnatures have not been completely characterized. [provided byRefSeq].
Background Reference 1:
Haurie, V., et al. Proc. Natl. Acad. Sci. U.S.A. 107(9):4176-4181(2010)
Background Reference 2:
Maitra, S., et al. RNA 14(5):950-959(2008)
Background Reference 3:
Leal, J.F., et al. Oncogene 27(14):1961-1970(2008)
Background Reference 4:
Yoo, N.K., et al. Cell. Microbiol. 10(3):583-592(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-998

List Size:
400 ul

List Price:

$495.00

Datasheet
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