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Western blot analysis of extracts of various cell lines, using BRMS1 antibody (15-766) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using BRMS1 antibody (15-766) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

BRMS1 Antibody

CATALOG NUMBER: 15-766

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human BRMS1 (NP_056214.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
Mouse kidney
Positive Control 2:
Mouse testis
Predicted Molecular Weight:
Observed: 28kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
BRMS1
Additional Names:
Breast cancer metastasis-suppressor 1, BRMS1
Ncbi Gene Id Number:
25855
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-766

List Size:
100 uL

List Price:

$436.00

Datasheet
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