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Western blot analysis of BRSK2 in human brain tissue lysate with BRSK2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of BRSK2 in human brain tissue with BRSK2 antibody at 5 μg/mL.
Immunofluorescence of BRSK2 in Human Brain cells with BRSK2 antibody at 20 μg/mL.
Western blot analysis of BRSK2 in human brain tissue lysate with BRSK2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Trial Size

BRSK2 Antibody

CATALOG NUMBER: 4085

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (87%)
Immunogen:
BRSK2 antibody was raised against a 23 amino acid synthetic peptide from near the carboxy terminus of human BRSK2.
The immunogen is located within amino acids 630 - 680 of BRSK2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
BRSK2 antibody can be used for detection of BRSK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
BRSK2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BRSK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BRSK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BRSK2
Additional Names:
BRSK2 Antibody: SAD1, SADA, STK29, PEN11B, C11orf7, HUSSY-12, Brain-selective kinase 2
Protein Accession Number:
Q8IWQ3
Protein Gi Number:
116241272
Ncbi Gene Id Number:
9024
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BRSK2 Antibody: BRSK2 was initially identified through a computer screen of the human genome and shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK2 is expressed in the brain and to a lesser extent in the testes. BRSK2 is a member of the AMP-activated protein kinase subfamily and can be activated by the tumor suppressor kinase LKB1. More recently, it has been shown that both BRSK2 and the related protein BRSK1 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. BRSK2 has also been shown to be an autoantigen in paraneoplastic limbic encephalitis. At least four isoforms of BRSK2 are known to exist.
Background Reference 1:
Manning G, Whyte DB, Martinez R, et al. The protein kinase complement of the human genome. Science2002; 298:1912-34.
Background Reference 2:
Lizcano JM, Goransson O, Toth R, et al. LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. EMBO J.2004; 23:833-43.
Background Reference 3:
Kishi M, Pan YA, Crump J, et al. Mammalian SAD kinases are required for neuronal polarization. Science2005; 307:929-32.
Background Reference 4:
Sabater L, Gomez-Choco M, Saiz A, et al. BR serine/threonine kinase 2: A new autoantigen in paraneoplastic limbic encephalitis. J. Neuroimmunol.2005; 170:186-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4085

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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