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Western blot analysis of extracts of various cell lines, using BTAF1 antibody (19-872) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody (19-872). Western blot was performed from the immunoprecipitate using BTAF1 antibody (19-872) at a dilition of 1:500.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using BTAF1 antibody (19-872) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of extracts of various cell lines, using BTAF1 antibody (19-872) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.

BTAF1 Antibody

CATALOG NUMBER: 19-872

Clonality:
Polyclonal
Tested Applications:
ChIP, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1).
Conjugate:
Unconjugated
Tested Applications:
ChIP, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:20 - 1:50
ChIP: 1:20 - 1:100
Positive Control 1:
HepG2
Positive Control 2:
HeLa
Positive Control 3:
MCF7
Positive Control 4:
Rat testis
Predicted Molecular Weight:
Observed: 250kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
BTAF1
Additional Names:
MOT1, TAF(II)17TAF172, TAFII17TATA-binding protein-associated factor 172, ATP-dependent helicase BTAF1, B-TFIID transcription factor-associated 170 kDa subunit, BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae), Mot1 homolog, TBP-associated factor 172
Ncbi Gene Id Number:
9044
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-872

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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