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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
BTC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of BTC Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

BTC Antibody

CATALOG NUMBER: 55-974

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human BTC.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
20 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BTC
Additional Names:
Probetacellulin, Betacellulin, BTC, BTC
Protein Accession Number:
P35070
Protein Gi Number:
461653
Ncbi Gene Id Number:
685
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the EGFfamily of growth factors. It is synthesized primarily as atransmembrane precursor, which is then processed to mature moleculeby proteolytic events. This protein is a ligand for the EGFreceptor.
Background Reference 1:
Stoeck, A., et al. J. Cell. Sci. 123 (PT 13), 2319-2331 (2010) :
Background Reference 2:
Genetos, D.C., et al. Cell Tissue Res. 340(1):81-89(2010)
Background Reference 3:
Nagaoka, T., et al. J. Mol. Biol. 380(1):83-94(2008)
Background Reference 4:
Revillion, F., et al. Ann. Oncol. 19(1):73-80(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-974

List Size:
400 ul

List Price:

$495.00

Datasheet
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