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Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
(LEFT)Western blot analysis of BTG1 Antibody in MCF-7 cell line lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of BTG1 Antibody in mouse NIH-3T3 tissue lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.
BTG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

BTG1 Antibody

CATALOG NUMBER: 61-795

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Rat
Immunogen:
This BTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-118 amino acids from the Central region of human BTG1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BTG1
Additional Names:
Protein BTG1, B-cell translocation gene 1 protein, BTG1
Protein Accession Number:
P62324
Protein Gi Number:
59799172
Ncbi Gene Id Number:
694
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BTG1 is a member of an anti-proliferative gene family that regulates cell growth and differentiation. Expression of this gene is highest in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. The encoded protein interacts with several nuclear receptors, and functions as a coactivator of cell differentiation. This locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia.
Background Reference 1:
Camina-Tato, M., et al. J. Neuroimmunol. 213 (1-2), 142-147 (2009) Zemunik, T., et al. Croat. Med. J. 50(1):23-33(2009) Takahashi, N., et al. Exp. Hematol. 36(12):1760-1770(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-795

List Size:
400 ul

List Price:

$495.00

Datasheet
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