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Western blot analysis using BTK monoclonal antibody against truncated BTK recombinant protein and K562 cell lysate.
Immunofluorescense analysis of Jurkat cells using BTK monoclonal antibody with FITC - IgG (top). Immunohistochemical analysis of paraffin - embedded human lymph follicle tissue showing cytoplasmic and membrane location using BTK antibody with DAB (bottom).
Western blot analysis using BTK monoclonal antibody against truncated BTK recombinant protein and K562 cell lysate.

BTK Antibody [7F12H4]

CATALOG NUMBER: 32-123

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant BTK expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
BTK monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BTK
Additional Names:
AGMX1, ATK, BPK, Agammaglobulinemia tyrosine kinase, AT, XLA, IMD1, AGMX1, PSCTK1
Protein Accession Number:
Q06187
Protein Gi Number:
547759
Ncbi Gene Id Number:
695
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
Background Reference 1:
Yamada, N., et al. Biochem. Biophys. Res. Commun. 192: 231-240.
Background Reference 2:
Thomas, J.D., et al. 1993. Science. 261: 355-358.
Background Reference 3:
Tamagnone, L., et al. Oncogene 9: 3683-3688.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-123

List Size:
0.1 mL

List Price:

$432.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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