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Western blot analysis of extracts of various cell lines, using BTK antibody (13-186) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using BTK antibody (13-186) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

BTK Antibody [9D11A9]

CATALOG NUMBER: 13-186

Clonality:
Monoclonal
Tested Applications:
IP, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein of human BTK
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:20 - 1:50
Positive Control 1:
K-562
Positive Control 2:
Raji
Positive Control 3:
THP-1
Positive Control 4:
Mouse spleen
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
BTK
Additional Names:
BTK Antibody: AT, ATK, BPK, XLA, IMD1, AGMX1, PSCTK1, Tyrosine-protein kinase BTK, Agammaglobulinemia tyrosine kinase
Ncbi Gene Id Number:
695
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-186

List Size:
50 uL

List Price:

$335.00

Datasheet
Shipping Info

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