BTLA Recombinant Protein

CATALOG NUMBER: 96-831

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Lys 31 - Ser 150
Fusion Tag:
Fc Tag
Tested Applications:
ELISA, WB
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Predicted Molecular Weight:
40.4 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human HVEM, His Tag at 5 ug/mL (100 uL/well) can bind Human BTLA (31-150), Fc Tag with a linear range of 0.08-1.25 ug/mL.
Purity:
>92% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
physical-state:
Lyophilized
Buffer:
Tris with Glycine, Arginine and NaCl, pH7.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
BTLA
Additional Names:
BTLA,CD272
Protein Accession Number:
AAP44003
Ncbi Gene Id Number:
151888
Background:
B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
Background Reference 1:
Watanabe N., et al., 2003, Nat. Immunol. 4:670-679.
Background Reference 2:
Gavrieli M., et al., 2003, Biochem. Biophys. Res. Commun. 312:1236-1243.
Background Reference 3:
Sedy J.R., et al., 2005, Nat. Immunol. 6:90-98.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-831

List Size:
0.1 mg

List Price:

$577.00

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