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Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 μg/mL.
Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 μg/mL.
Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 μg/mL.
Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 μg/mL.
Trial Size

Bub1 Antibody

CATALOG NUMBER: 4229

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.
The immunogen is located within amino acids 40 - 90 of Bub1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Purification:
Bub1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bub1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BUB1
Additional Names:
Bub1 Antibody: BUB1A, BUB1L, hBUB1, BUB1A
Protein Accession Number:
O43683
Protein Gi Number:
8134347
Ncbi Gene Id Number:
699
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bub1 Antibody: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
Background Reference 1:
Pangilinan F, Li Q, Weaver T, et al. Mammalian BUB1 protein kinases: map positions and in vivo expression. Genomics1997; 46:379-88.
Background Reference 2:
Williams GL, Roberts TM and Gjoerup OV. Bub1: Escapades in a cellular world. Cell Cycle2007; 6:1699-704.
Background Reference 3:
Meraldi P and Sorger PK. A dual role for Bub1 in the spindle checkpoint and chromosome congression. EMBO J.2005; 24:1621-33.
Background Reference 4:
Tang Z, Shu H, Oncel D, et al. Phosphorylation of cdc20 by bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol. Cell2004; 16:387-97.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4229

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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