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Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-alpha (phospho-Ser21) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-alpha (phospho-Ser21) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-alpha (phospho-Ser21) Antibody. The lane on the right is treated with the antigen-specific peptide.

C/EBP-alpha (phospho Ser21) Antibody

CATALOG NUMBER: 79-780

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
C/EBP-alpha (phospho Ser21) antibody was raised against a peptide sequence around phosphorylation site of Serine 21 (L-Q-S (p)-P-P) derived from Human C/EBP-alpha.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at serine 21.
Predicted Molecular Weight:
45 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CEBPA
Additional Names:
CAP4, ICE8, MACH, MCH5, Mch-5
Protein Accession Number:
NP_004355.2
Protein Gi Number:
28872794
Ncbi Gene Id Number:
1050
Background:
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Background Reference 1:
Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461.
Background Reference 2:
Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194 - 1204.
Background Reference 3:
Raphaël Métivier, Mol. Endocrinol., Nov 2001; 15: 1953.
Background Reference 4:
Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-780

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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