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Western blot analysis of extracts from cos-7 cells (Lane 2), using C/EBP-alpha (Phospho-Thr230) Antibody. The lane on the left is treated with antigen-specific peptide.
Western blot analysis of extracts from cos-7 cells (Lane 2), using C/EBP-alpha (Phospho-Thr230) Antibody. The lane on the left is treated with antigen-specific peptide.

C/EBP-alpha (phospho Thr230) Antibody

CATALOG NUMBER: 79-898

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
C/EBP-alpha (phospho Thr230) antibody was raised against a peptide sequence around phosphorylation site of threonine 230 (P-P-T (p)-P-V) derived from Human C/EBP-alpha.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The Antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at Thr230.
Predicted Molecular Weight:
42 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CEBPA
Additional Names:
CCAAT/enhancer-binding protein alpha C/EBP alpha CEBPA
Protein Accession Number:
NP_004355.2
Protein Gi Number:
28872794
Ncbi Gene Id Number:
1050
Background:
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.
Background Reference 1:
Xanthopoulos K.G. Biochem. Biophys. Res. Commun. 215:106-113(1995)
Background Reference 2:
Schalwijk J. Biol. Chem. Hoppe-Seyler 378:373-379(1997)
Background Reference 3:
SeattleSNPs variation discovery resource Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-898

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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