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Western blot analysis of lysed extracts from A549, HeLa and 3T3 cells using C-Fos Antibody.
Western blot analysis of lysed extracts from A549, HeLa and 3T3 cells using C-Fos Antibody.

C Fos Antibody

CATALOG NUMBER: 79-766

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
C-Fos antibody was raised against a peptide sequence around aa.28~32 ( S-Y-Y-H-S) derived from Human C-Fos.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of total C-Fos protein.
Predicted Molecular Weight:
62 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
FOS
Additional Names:
p55, AP-1, C-FOS, p55
Protein Accession Number:
NP_005243.1
Protein Gi Number:
4885241
Ncbi Gene Id Number:
2353
Background:
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
Background Reference 1:
Okazaki K., Sagata N. EMBO J. 14:5048-5059 (1995) Zhang Y., Feng X. H., Derynck R. Nature 394:909-913 (1998) Bossis G., Malnou C. E., Farras R., Andermarcher E., Hipskind R., Rodriguez M., Schmidt D., Muller S., Jariel-Encontre I., Piechaczyk M. Mol. Cell. Biol. 25:6964-6979 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-766

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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