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Western blot analysis of extracts of various cell lines, using c-Jun antibody (14-004) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using Histone H2AX antibody (13-997) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using c-Jun antibody (14-004) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using c-Jun antibody (14-004) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using c-Jun antibody (14-004) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

c-Jun Antibody

CATALOG NUMBER: 14-004

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human c-Jun (NP_002219.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HT-1080
Positive Control 2:
Mouse lung
Positive Control 3:
Rat lung
Predicted Molecular Weight:
Observed: 43kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Jun
Additional Names:
JUN, AP1, AP-1, c-Jun
Ncbi Gene Id Number:
3725
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-004

List Size:
100 uL

List Price:

$436.00

Datasheet
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