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Western blot analysis of lysed extracts from PC14 cells untreated or treated with EGF using c-Jun (Phospho-Ser73).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Phospho-Ser73).
Western blot analysis of lysed extracts from PC14 cells untreated or treated with EGF using c-Jun (Phospho-Ser73).

c Jun (phospho Ser73) Antibody

CATALOG NUMBER: 79-102

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
c-Jun (Phospho-Ser73) antibody was raised against a peptide sequence around phosphorylation site of serine 73 (L-A-S (p) -P-E) derived from Human c-Jun.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of c-Jun only when phosphorylated at serine 73.
Predicted Molecular Weight:
43 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
JUN
Additional Names:
AP1, AP-1, c-Jun, MEK2, MKK2, PRKMK2, AH119, AP1, Jun A, p39
Protein Accession Number:
NP_002219.1
Protein Gi Number:
4758616
Ncbi Gene Id Number:
3725
Background:
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
Background Reference 1:
Sevilla A, et al. (2004) Oncogene. 23 (55): 8950-8958. Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101 (33): 12130-12135. Binetruy B, et al. (1991) Nature. 351:122-127. Smeal T, et al. (1991) Nature. 354: 494-496. Derijard B, et al. (1994) Cell. 76:1025-1037.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-102

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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