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Western blot analysis of lysed extracts from HeLa cells untreated or treated with IFN-α using c-Kit (Phospho-Tyr721).
Western blot analysis of lysed extracts from HeLa cells untreated or treated with IFN-α using c-Kit (Phospho-Tyr721).

c Kit (phospho Tyr721) Antibody

CATALOG NUMBER: 79-480

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
c-Kit (Phospho-Tyr721) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 721 (N-E-Y (p) -M-D) derived from Human c-Kit.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of c-Kit only when phosphorylated at tyrosine 721.
Predicted Molecular Weight:
145 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
KIT
Additional Names:
PBT, SCFR, C-Kit, CD117, CTNNG, DP3,
Protein Accession Number:
NP_000213.1
Protein Gi Number:
4557695
Ncbi Gene Id Number:
3815
Background:
This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)
Background Reference 1:
Nocka, K. et al. (1990) EMBO J. 9, 1805-1813. Hirota, S. et al. (1998) Science 279, 577-580. Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157-162. Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248-10253. Gommerman, J. L. et al. (1997) J. Biol. Chem. 272, 30519-30525.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-480

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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