Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of C-raf in A431 cell lysate with NPC1 antibody at (A) 0.5 and (B) 1 μg/mL.
Western blot analysis of C-raf in A431 cell lysate with NPC1 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

C-raf Antibody

CATALOG NUMBER: 5779

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (84%)
Immunogen:
C-raf antibody was raised against an 19 amino acid synthetic peptide near the amino terminus of human C-raf.
The immunogen is located within amino acids 30 - 80 of C-raf.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
C-raf antibody can be used for detection of C-raf by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Predicted Molecular Weight:
Predicted: 72 kDa
Observed: 72 kDa
Purification:
C-raf Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
C-raf Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
C-raf antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RAF1
Additional Names:
C-raf Antibody: NS5, CRAF, Raf-1, c-Raf, RAF, Proto-oncogene c-RAF, cRaf
Protein Accession Number:
NP_002871
Protein Gi Number:
4506401
Ncbi Gene Id Number:
5894
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
C-raf Antibody: C-raf is the cellular homolog of viral raf gene (v-raf) and encodes a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases. Once activated, C-raf phosphorylates and activates the protein kinases MEK1 and MEK2, which in turn phosphorylate and activate the serine/threonine specific protein kinases, ERK1 and ERK2. These activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Background Reference 1:
Howe LR, Leevers SJ, Gomez N, et al. Activation of the MAP kinase pathway by the protein kinase raf. Cell1992; 71:335-42.
Background Reference 2:
Chang F, Steelman LS, Shelton JG, et al. Regulation of cell cycle progression and apoptosis by the Ras/Raf/MEK/ERK pathway. Int. J. Oncol.2003; 22:469-80.
Background Reference 3:
Pandit B, Sarkozy A, Pennacchio LA, et al. Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy. Nat. Genet.2007; 39:1007-12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5779

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369