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Western blot analysis of C10orf27 Antibody in Ramos cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with C10orf27 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of C10orf27 Antibody in Ramos cell line lysates (35ug/lane)

C10orf27 Antibody

CATALOG NUMBER: 63-590

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This C10orf27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-302 amino acids from the C-terminal region of human C10orf27.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TBATA
Additional Names:
Protein TBATA, Protein SPATIAL, Stromal protein associated with thymii and lymph node homolog, Thymus, brain and testes-associated protein, TBATA, C10orf27, SPATIAL
Protein Accession Number:
Q96M53
Protein Gi Number:
317373509
Ncbi Gene Id Number:
219793
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
C10orf27 may play a role in spermatid differentiation.
Background Reference 1:
Goertsches,R., et.al., Mult. Scler. 14 (3), 412-414 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-590

List Size:
400 ul

List Price:

$495.00

Datasheet
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