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Western blot analysis in HL-60 cell line lysates (35ug/lane).
C1QC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of C1QC Antibody with WiDr cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in HL-60 cell line lysates (35ug/lane).

C1QC Antibody

CATALOG NUMBER: 56-126

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This C1QC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the Central region of human C1QC.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
26 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
C1QC
Additional Names:
Complement C1q subcomponent subunit C, C1QC, C1QG
Protein Accession Number:
P02747
Protein Gi Number:
20178281
Ncbi Gene Id Number:
714
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a major constituent of the humancomplement subcomponent C1q. C1q associates with C1r and C1s inorder to yield the first component of the serum complement system.A deficiency in C1q has been associated with lupus erythematosusand glomerulonephritis. C1q is composed of 18 polypeptide chains:six A-chains, six B-chains, and six C-chains. Each chain contains acollagen-like region located near the N-terminus, and a C-terminalglobular region. The A-, B-, and C-chains are arranged in the orderA-C-B on chromosome 1. This gene encodes the C-chain polypeptide ofhuman complement subcomponent C1q. Alternatively spliced transcriptvariants that encode the same protein have been found for thisgene.
Background Reference 1:
Fraser, D.A., et al. J. Immunol. 185(7):3932-3939(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Rafiq, S., et al. Clin. Exp. Immunol. 161(2):284-289(2010)
Background Reference 4:
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-126

List Size:
400 ul

List Price:

$495.00

Datasheet
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