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Western blot analysis of C2CD3 in EL4 cell lysate with C2CD3 antibody at (A) 1 and (B) 2 μg/ml.
Western blot analysis of C2CD3 in EL4 cell lysate with C2CD3 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

C2CD3 Antibody

CATALOG NUMBER: 8169

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
C2CD3 antibody was raised against an 18 amino acid peptide near the center of human C2CD3.
The immunogen is located within amino acids 1490 - 1540 of C2CD3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
C2CD3 antibody can be used for detection of C2CD3 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity:
C2CD3 antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Predicted Molecular Weight:
Predicted: 216, 259 kDa
Observed: 255 kDa
Purification:
C2CD3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
C2CD3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
C2CD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
C2CD3
Additional Names:
C2 calcium-dependent domain containing 3, C2 domain-containing protein 3
Protein Accession Number:
NP_001273506
Protein Gi Number:
148596944
Ncbi Gene Id Number:
26005
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
C2CD3, a putative calcium-dependent lipid-binding protein, is an essential regulator of intracellular transduction of the Hedgehog signal (1). It is localized at the basal body of cilia, mediates calcium-dependent vesicular transport and/or recruitment of proteins and regulates cilium biogenesis by promoting the assembly of centriolar distal appendages critical for docking ciliary vesicles and recruiting other essential ciliogenic proteins (2,3). C2CD3 directly promotes centriole elongation and OFD1 acts as a negative regulator of C2CD3 (4,5).
Background Reference 1:
Hoover AN, Wynkoop A, Zeng H, et al. C2cd3 is required for cilia formation and Hedgehog signaling in mouse. Development 2008; 135:4049-58.
Background Reference 2:
Ye X, Zeng H, Ning G, et al. C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals. Proc. Natl. Acad. Sci. USA 2014; 111:2164-9.
Background Reference 3:
Thauvin-Robinet C, Lee JS, Lopez E, et al. The oral-facial-digital syndrome gene C2CD3 encodes a positive regulator of centriole elongation. Nat. Genet. 2014; 46:905-11.
Background Reference 4:
Balestra FR, Strnad P, Fluckiger I, et al. Discovering regulators of centriole biogenesis through siRNA-based functional genomics in human cells. Dev. Cell 2013; 25:555-71.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8169

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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