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Antibody used in WB on Mouse NIH-3T3 at 0.125 ug/ml.
Antibody used in WB on Mouse NIH-3T3 at 0.125 ug/ml.

C2TA Antibody

CATALOG NUMBER: 28-570

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse C2TA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
C2TA antibody can be used for detection of C2TA by ELISA at 1:62500. C2TA antibody can be used for detection of C2TA by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Predicted Molecular Weight:
119 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store C2TA antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
Ciita
Additional Names:
C2TA, C2ta, Gm9475, EG669998
Protein Accession Number:
NP_031601
Protein Gi Number:
6671654
Ncbi Gene Id Number:
12265
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
C2ta is essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. C2ta may act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter
Background Reference 1:
Pennini, M.E., et al., (2006) J. Immunol. 176 (7), 4323-4330.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-570

List Size:
100 ul

List Price:

$519.00

Datasheet
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