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43-098 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
43-098 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-098 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

C3 Antibody

CATALOG NUMBER: 43-098

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: QQRTFISPIKCRE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 125kDa band in Human Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta and Testis. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize the C3 alpha chain, C3b alpha' chain and the C3c alpha' chain fragment 2.
Predicted Molecular Weight:
Approx 125 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
C3
Additional Names:
C3, complement component 3, AHUS5, ARMD9, ASP, CPAMD1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, OTTHUMP00000197086, acylation-stimulating protein cleavage product, complement C3, complement component C3
Protein Accession Number:
NP_000055.2
Protein Gi Number:
115298678
Ncbi Gene Id Number:
718

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-098

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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