C4orf14 Antibody

CATALOG NUMBER: 42-047

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat
Immunogen:
The immunogen for this antibody is: ESDYCTAKGSEAIDR
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Breast and in Breast cancer lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.5kDa according to NP_115689.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
C4orf14
Additional Names:
NOA1, nitric oxide associated 1, C4orf14, hAtNOS1, hNOA1, mAtNOS1, nitric oxide associated-1, nitric oxide synthase, mitochondrial (putative), nitric oxide-associated protein 1, putative ortholog of Arabidopsis mitochondrial nitric oxide synthase
Protein Accession Number:
NP_115689.1
Protein Gi Number:
14150078
Ncbi Gene Id Number:
84273
Background Reference 1:
Parihar A, Parihar MS, Chen Z, Ghafourifar P. mAtNOS1 induces apoptosis of human mammary adenocarcinoma cells. Life Sci. 2008 May 23;82(21-22):1077-82.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-047

List Size:
0.1 mg

List Price:

$369.00

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