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Western blot analysis in A549 cell line lysates (35ug/lane).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549 cell line lysates (35ug/lane).

C5AR1 Antibody

CATALOG NUMBER: 55-354

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This C5AR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-201 amino acids from the Central region of human C5AR1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
C5AR1
Additional Names:
C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88, C5AR1, C5AR, C5R1
Protein Accession Number:
P21730
Protein Gi Number:
311033355
Ncbi Gene Id Number:
728
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Background Reference 1:
Skeie, J.M., et al. Invest. Ophthalmol. Vis. Sci. 51(10):5336-5342(2010)
Background Reference 2:
Nishiura, H., et al. Apoptosis 15(8):966-981(2010)
Background Reference 3:
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Background Reference 4:
Marc, M.M., et al. Scand. J. Immunol. 71(5):386-391(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-354

List Size:
400 ul

List Price:

$495.00

Datasheet
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