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Western blot analysis of C7 Antibody in Ramos cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lymph tissue reacted with C7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of C7 Antibody in Ramos cell line lysates (35ug/lane)

C7 Antibody

CATALOG NUMBER: 63-861

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This C7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 375-403 amino acids from the Central region of human C7.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
94 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
C7
Additional Names:
Complement component C7, C7
Protein Accession Number:
P10643
Protein Gi Number:
61252057
Ncbi Gene Id Number:
730
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
C7 is a component of the complement system. It participates in the formation of Membrane Attack Complex (MAC). People with C7 deficiency are prone to bacterial infection.
Background Reference 1:
Davila,S., et.al., Genes Immun. 11 (3), 232-238 (2010)
Background Reference 2:
Kuijpers,T.W., et.al., Mol. Immunol. 47 (4), 671-677 (2010)
Background Reference 3:
Wheeler,H.E., et.al., PLoS Genet.(10), E1000685 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-861

List Size:
400 ul

List Price:

$495.00

Datasheet
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