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Western blot analysis of CA1 using rabbit polyclonal CA1 Antibody. HEK293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA1 gene.
CA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HEK293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CA1 using rabbit polyclonal CA1 Antibody. HEK293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA1 gene.

CA1 Antibody

CATALOG NUMBER: 56-562

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the N-terminal region of human CA1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CA1
Additional Names:
Carbonic anhydrase 1, Carbonate dehydratase I, Carbonic anhydrase B, CAB, Carbonic anhydrase I, CA-I, CA1
Protein Accession Number:
P00915
Protein Gi Number:
115449
Ncbi Gene Id Number:
759
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Carbonic anhydrases (CAs) are a large family of zincmetalloenzymes that catalyze the reversible hydration of carbondioxide. They participate in a variety of biological processes,including respiration, calcification, acid-base balance, boneresorption, and the formation of aqueous humor, cerebrospinalfluid, saliva, and gastric acid. They show extensive diversity intissue distribution and in their subcellular localization. CA1 isclosely linked to CA2 and CA3 genes on chromosome 8, and it encodesa cytosolic protein which is found at the highest level inerythrocytes. Variants of this gene have been described in somepopulations. Multiple alternatively spliced variants, encoding thesame protein, have been identified. Transcript variants of CA1utilizing alternative polyA_sites have been described inliterature.
Background Reference 1:
Abhary, S., et al. Mol. Vis. 15, 1179-1184 (2009) :
Background Reference 2:
Gambhir, K.K., et al. Biochem. Genet. 45 (5-6), 431-439 (2007) :
Background Reference 3:
Temperini, C., et al. Bioorg. Med. Chem. Lett. 17(8):2210-2215(2007)
Background Reference 4:
Temperini, C., et al. Bioorg. Med. Chem. Lett. 16(19):5152-5156(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-562

List Size:
400 ul

List Price:

$495.00

Datasheet
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