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Western blot analysis of CA14 antibody in A375 cell line lysates (35ug/lane)
Flow cytometric analysis of A375 cells using CA14 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CA14 using rabbit polyclonal CA14 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA14 gene.
Western blot analysis of CA14 antibody in A375 cell line lysates (35ug/lane)

CA14 Antibody

CATALOG NUMBER: 62-807

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CA14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 amino acids from the N-terminal region of human CA14.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CA14
Additional Names:
Carbonic anhydrase 14, Carbonate dehydratase XIV, Carbonic anhydrase XIV, CA-XIV, CA14
Protein Accession Number:
Q9ULX7
Protein Gi Number:
8928036
Ncbi Gene Id Number:
23632
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CA14 belongs a large family of zinc metalloenzymes that catalyze the versible hydration of carbon dioxide. The protein is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles.
Background Reference 1:
Parkkila,S. Natl. Acad. Sci. U.S.A. 98 (4), 1918-1923 (2001)
Background Reference 2:
Fujikawa-Adachi,K., Nishimori,I. Genomics 61 (1), 74-81 (1999)
Background Reference 3:
Tarun,A.S., Bryant,B. Chem. Senses 28 (7), 621-629 (2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-807

List Size:
400 ul

List Price:

$495.00

Datasheet
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