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Western blot analysis of CA9 antibody in Hela cell line lysates (35ug/lane)
Flow cytometric analysis of hela cells using CA9 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescence analysis of CA9 Antibody with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Western blot analysis of CA9 antibody in Hela cell line lysates (35ug/lane)

CA9 Antibody

CATALOG NUMBER: 61-750

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-143 amino acids from the Central region of human CA9.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CA9
Additional Names:
Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, CAIX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, RCC-associated antigen G250, pMW1, CA9, G250, MN
Protein Accession Number:
Q16790
Protein Gi Number:
83300925
Ncbi Gene Id Number:
768
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
Background Reference 1:
Grabmaier, K., et al., Oncogene 23(33):5624-5631 (2004).
Background Reference 2:
Kaluzova, M., et al., Mol. Cell. Biol. 24(13):5757-5766 (2004).
Background Reference 3:
Span, P.N., et al., Br. J. Cancer 89(2):271-276 (2003).
Background Reference 4:
Hedley, D., et al., Clin. Cancer Res. 9(15):5666-5674 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-750

List Size:
400 ul

List Price:

$495.00

Datasheet
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