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Western blot analysis of CACNA1H in 293 cell lysate with CACNA1H antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of CACNA1H in mouse brain tissue with CACNA1H antibody at 5 μg/ml.
Immunofluorescence of CACNA1H in mouse brain tissue with CACNA1H antibody at 20 μg/ml.
Western blot analysis of CACNA1H in 293 cell lysate with CACNA1H antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

CACNA1H Antibody

CATALOG NUMBER: 8029

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CACNA1H antibody was raised against a 16 amino acid peptide near the center of human CACNA1H.
The immunogen is located within amino acids 1010 - 1060 of CACNA1H.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CACNA1H antibody can be used for detection of CACNA1H by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
CACNA1H antibody is human, mouse and rat reactive. Multiple isoforms of CACNA1H are known to exist.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Predicted Molecular Weight:
Predicted: 259 kDa
Observed: 260 kDa
Purification:
CACNA1H antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CACNA1H antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CACNA1H antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CACNA1H
Additional Names:
Calcium channel voltage-dependent T type alpha 1H subunit, CACNA1HB, Cav3.2, ECA6, EIG6
Protein Accession Number:
NP_066921
Protein Gi Number:
53832009
Ncbi Gene Id Number:
8912
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The calcium channel protein CACNA1H is a T-type member of the alpha-1 subunit family that is part of the voltage-dependent calcium channel complex which mediates the influx of calcium ions into the cell upon membrane polarization (1,2). CACNA1H is a subunit of Cav3.2 T-type calcium channel that is involved in neurological disorders such as epilepsy and pain (2,3). CACNA1H associates with the caveolin protein Cav-3 and it is thought that Cav-3 regulates the Protein Kinase A (PKA) modulation of CACNA1H-containing Cav3.2 T-type calcium channels (4).
Background Reference 1:
Cribbs LL, Lee JH, Yang J, et al. Cloning and characterization of alpha1H from human heart, a member of the T-type CA2+ channel gene family. Circ. Res. 1998; 83:103-9.
Background Reference 2:
Sekiguchi F and Kawabata A. T-type calcium channels: functional regulation and implication in pain signaling. J. Pharmacol. Sci. 2013; 122:244-50.
Background Reference 3:
McGivern JG. Targeting N-type and T-type calcium channels for the treatment of pain. Drug Discov. Today 2006; 11:245-53.
Background Reference 4:
Markandeya YS, Fahey JM, Pluteanu F, et al. Caveolin-3 regulates protein kinase A modulation of the Cav3.2 (a1H) T-type Ca2+ channels. J. Biol. Chem. 2011; 286:2433-2444.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8029

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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