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Western Blot at 1:2000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + mouse kidney lysate Lysates/proteins at 20 ug per lane.
Fluorescent image of U251 cell stained with CACNA2D2 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CACNA2D2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).CACNA2D2 immunoreactivity is localized to Cytoplasm significantly.
CACNA2D2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:2000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.

CACNA2D2 Antibody

CATALOG NUMBER: 57-021

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CACNA2D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 643-671 amino acids from the Central region of human CACNA2D2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
130 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CACNA2D2
Additional Names:
Voltage-dependent calcium channel subunit alpha-2/delta-2, Voltage-gated calcium channel subunit alpha-2/delta-2, Voltage-dependent calcium channel subunit alpha-2-2, Voltage-dependent calcium channel subunit delta-2, CACNA2D2, KIAA0558
Protein Accession Number:
Q9NY47
Protein Gi Number:
387912827
Ncbi Gene Id Number:
9254
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.
Background Reference 1:
Donato, R., et al. J. Neurosci. 26(48):12576-12586(2006) Ivanov, S.V., et al. Am. J. Pathol. 165(3):1007-1018(2004) Carboni, G.L., et al. Oncogene 22(4):615-626(2003) Honorio, S., et al. Mol. Cell. Probes 15(6):391-393(2001) Angeloni, D., et al. Mol. Cell. Probes 15(2):125-127(2001)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-021

List Size:
400 ul

List Price:

$519.00

Datasheet
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